Obtaining 3-D, Full-Color Images Using A Microscope

Must read

NextGen SCADA 2021

Across the globe, utilities are looking to advance their grid management and control techniques. The critical infrastructure that coordinates the energy system, SCADA, EMS,...

3D PluraView and Vesalius3D for Medical 3D Visualizations

Understanding a patient’s specific anatomy is of key importance for best medical results. Fast and efficient interpretation of medical imaging is essential.The high-resolution...

Choosing the Landing Site of the Mars Perseverance Rover with Remote Sensing

Mars 2020 is a Mars rover mission by NASA's Mars Exploration Program that includes the rover Perseverance and the small robotic helicopter Ingenuity. Mars 2020 was launched from Earth on 30 July 2020,...

Smart Cities and Their Digital Assets

What is the development benefit of the smart city for the city in the future? This is a problem worthy of attention to the...
Nursinem Handan ŞAHAN
2018 yılında Yıldız Teknik Üniversitesi Harita Mühendisliği bölümünü onur öğrencisi olarak tamamladı. Lisans eğitimi sırasında Erasmus+ programıyla Varşova Teknoloji Üniversitesinde öğrenim gördü. Halihazırda öğrenimine İstanbul Teknik Üniversitesi Coğrafi Bilgi Teknolojileri bölümünde devam etmekte.

Conventional wide-field microscopy (WFM) cannot provide optical sectioning (OS) images that are required for 3-D volumetric reconstruction. The reason lies in the fact that the out-of-focus signals are always coupled within the in-focus plane. By introducing structured illumination microscopy (SIM), researchers have achieved removing the out-of-focus components from the in-focus plane in full color (FC).

However, the current FC-SIM approach needs three phase-shifted raw images for each focused plane and hundreds of axial-scanned planes. This approach causes a significant number of raw images and thus putting a heavy burden on data storage and processing time cost. How to release such a burden is still a question.

Recently, a research team led by Prof. Yao Baoli at State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics (XIOPM) of the Chinese Academy of Sciences (CAS), has reported a deep learning scheme FC-WFM-Deep to obtain full-color optical sectioning images for wide-field microscopy directly.

FC-WFM-Deep fully exploits the unique high resolution and full-color capabilities of SIM. Efficiently, the deep learning network only needs training to a single wide-field frame. After training, high-quality images with optical sectioning, considerable in-focus depth, and full-color can be directly acquired from the wide-field frame.

FC-WFM-Deep has a comparable imaging quality to FC-SIM in terms of spatial resolution and dimensions. Beyond that, the data required for FC-WFM-Deep in full-color 3-D reconstruction can be 21-fold less than that for FC-SIM.

FC-WFM-Deep significantly reduces the 3-D data acquisition requirements without losing detail and improves the 3-D imaging speed by extracting the optical sectioning in the depth-of-field (DOF). This cost-effective and convenient method offers a promising tool to observe 3-D color biological samples with high precision.

Source: Researchers directly obtain 3-D, full-color images with conventional microscope

More articles

LEAVE A REPLY

Please enter your comment!
Please enter your name here

- Advertisement -

Latest article

NextGen SCADA 2021

Across the globe, utilities are looking to advance their grid management and control techniques. The critical infrastructure that coordinates the energy system, SCADA, EMS,...

3D PluraView and Vesalius3D for Medical 3D Visualizations

Understanding a patient’s specific anatomy is of key importance for best medical results. Fast and efficient interpretation of medical imaging is essential.The high-resolution...

Choosing the Landing Site of the Mars Perseverance Rover with Remote Sensing

Mars 2020 is a Mars rover mission by NASA's Mars Exploration Program that includes the rover Perseverance and the small robotic helicopter Ingenuity. Mars 2020 was launched from Earth on 30 July 2020,...

Smart Cities and Their Digital Assets

What is the development benefit of the smart city for the city in the future? This is a problem worthy of attention to the...

7 Benefits of Using Remote Sensing & GIS in Agriculture

Remote sensing and Geographic Information System (GIS) technologies are commonly used for real-time analysis and forecasts of weather, floods, and monitoring crop area sown,...